This article explores the latest bioinformatics advancements that enable enhanced base calling, base modification detection, error correction, assembly, and alignment of ONT data.
Long-Read knowledge: Nanopore Full-Length Transcriptome Sequencing
Nanopore Sequencing enables to accurately identify various structural variations within transcripts, including alternative splicing, gene fusion, selective polyadenylation of APAs, allele-specific expression, and other alterations in transcript structure.